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  Indian J Med Microbiol
 

Figure 7: Annexin V-PI expression study of the test compound 1 (Methanolic leaf extract) against A549 cell line: Annexin V-PI expression study of test compounds on A549 cells using BD FACS Calibur, Cell Quest Pro Software (Version 6.0). Quadrants showing the expression of Annexin V-PI in untreated cells (a), 25 μM cisplatin treated cells (b) and Calotropis gigantea methanolic leaf extract treated cells (c). In each figure, the upper left quadrant indicates the percentage (%) of necrotic cells and the upper right quadrant indicates the percentage (%) of late apoptotic cells. Bottom left quadrant indicates the percentage (%) of viable cells and bottom right indicates the percentage (%) of early apoptotic cells

Figure 7: Annexin V-PI expression study of the test compound 1 (Methanolic leaf extract) against A549 cell line: Annexin V-PI expression study of test compounds on A549 cells using BD FACS Calibur, Cell Quest Pro Software (Version 6.0). Quadrants showing the expression of Annexin V-PI in untreated cells (a), 25 μM cisplatin treated cells (b) and Calotropis gigantea methanolic leaf extract treated cells (c). In each figure, the upper left quadrant indicates the percentage (%) of necrotic cells and the upper right quadrant indicates the percentage (%) of late apoptotic cells. Bottom left quadrant indicates the percentage (%) of viable cells and bottom right indicates the percentage (%) of early apoptotic cells