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  Indian J Med Microbiol
 

Figure 1: (a) Specific Omp 28 gene PCR of S.Typhi MTCC 733, Lane 1: Omp 28 gene PCR on S.Typhi MTCC 733, Lane M: Low range DNA ruler Plus(Genei), (B) Colony PCR for screening of recombinant clones, Lane 1: Negative Control, Lane 2: Positive Control, Lane 3-7: PCR amplicon of Clones, Lane M: Low range DNA ruler Plus(Genei), (C) Restriction Enzyme Digestion with Not I and Xba I to release insert, Lane 1: Positive amplicon of selected clone, Lane M: Low range DNA ruler plus(Gene), Lane 2: Digested Plasmid with Not I and Xba I to release insert

Figure 1: (a) Specific Omp 28 gene PCR of S.Typhi MTCC 733, Lane 1: Omp 28 gene PCR on S.Typhi MTCC 733, Lane M: Low range DNA ruler Plus(Genei), (B) Colony PCR for screening of recombinant clones, Lane 1: Negative Control, Lane 2: Positive Control, Lane 3-7: PCR amplicon of Clones, Lane M: Low range DNA ruler Plus(Genei), (C) Restriction Enzyme Digestion with Not I and Xba I to release insert, Lane 1: Positive amplicon of selected clone, Lane M: Low range DNA ruler plus(Gene), Lane 2: Digested Plasmid with Not I and Xba I to release insert