Journal of Natural Science, Biology and Medicine

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 10  |  Issue : 3  |  Page : 131--135

Evaluation of sanger sequencing method for ESR1 mutation detection in primary breast tumor


Ramadhan Karsono1, Adhitya Bayu Perdana3, Fahreza Saputra3, Yulia Pratiwi2, Ayu Nurdiantika Sari3, Abi Abinawanto3 
1 Department of Surgical Oncology; Department of Research and Development, Dharmais Hospital-National Cancer Center, Jakarta, Indoneisa
2 Functional Medical Staff of Surgical Oncology Department, Dharmais Hospital-National Cancer Center, Jakarta, Indoneisa
3 Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Jakarta, Indoneisa

Correspondence Address:
Ramadhan Karsono
Departement of Surgical Oncology, Dharmais Hospital-National Cancer Center, Jakarta
Indoneisa

Objective: Exon 8 estrogen receptor 1 (ESR1) mutations in the ligand-binding domain play an important role in mechanisms of hormonal therapy resistance in breast cancer. Identification of ESR1 mutations is very important in determining the appropriate steps of therapy. In this study, we evaluate Sanger sequencing technique to detect the ESR1 mutation. Methods: This retrospective study was conducted using 49 advanced breast cancer patients with estrogen receptor positive. Deoxyribonucleic acid (DNA) material was extracted from primary breast tumor samples. Exon 8 ESR1 gene mutation was analyzed by Sanger sequencing method using BigDye Direct Sequencing Kit (Applied Biosystem) with gBlock synthesis gene fragment (Integrated DNA Technologies) D538G as a positive control. Results: The mean age of patients was 46.14 (±9.6) years, and 61.2% were in stage 4. There are no exon 8 ESR1 gene mutations detected in 49 primary tumor samples, whereas the gBlock-positive control showed base substitution in 1613A>G (D538G) indicating the success of sequencing reaction. Conclusion: Sanger sequencing has failed to detect ESR1 mutation in primary tumor breast samples. Other advanced molecular techniques should be performed for diagnosis of primary breast tumors.


How to cite this article:
Karsono R, Perdana AB, Saputra F, Pratiwi Y, Sari AN, Abinawanto A. Evaluation of sanger sequencing method for ESR1 mutation detection in primary breast tumor.J Nat Sc Biol Med 2019;10:131-135


How to cite this URL:
Karsono R, Perdana AB, Saputra F, Pratiwi Y, Sari AN, Abinawanto A. Evaluation of sanger sequencing method for ESR1 mutation detection in primary breast tumor. J Nat Sc Biol Med [serial online] 2019 [cited 2020 Nov 24 ];10:131-135
Available from: http://www.jnsbm.org/article.asp?issn=0976-9668;year=2019;volume=10;issue=3;spage=131;epage=135;aulast=Karsono;type=0