ORIGINAL ARTICLE
Year : 2018  |  Volume : 9  |  Issue : 1  |  Page : 47-53

In vitro H+/K+-ATPase Inhibition, Antiradical effects of a flavonoid-rich fraction of Dissotis rotundifolia, and In silico PASS Prediction of its isolated compounds


1 Department of Biochemistry, School of Biological Sciences, College of Agriculture and Natural Sciences, University of Cape Coast, Cape Coast, Ghana
2 Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
3 Department of Molecular Biology and Biotechnology, School of Biological Sciences, College of Agriculture and Natural Sciences, University of Cape Coast, Cape Coast, Ghana
4 Department of Biochemistry, School of Biological Sciences, College of Agriculture and Natural Sciences, University of Cape Coast, Cape Coast, Ghana; Department of Biochemistry, Virginia Polytechnic Institute and State University, Virginia, USA
5 Department of Pharmacology, Faculty of Pharmacy, University of , Legon, Accra, Ghana

Correspondence Address:
Michael Buenor Adinortey
Department of Biochemistry, University of Cape Coast, Cape Coast
Ghana
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jnsbm.JNSBM_104_17

Rights and Permissions

Background: Dissotis rotundifolia, commonly referred to as pink lady, has several medicinal uses including peptic ulcer. This study investigated the inhibitory effects of D. rotundifolia extract on H+/K+-ATPase and also assessed its antiradical activity. In silico study of some isolated compounds of this plant was also carried out to affirm the suspected binding properties of extract to H+/K+-ATPase enzyme. Materials and Methods: D. rotundifolia whole plant extract was obtained after extraction process and then assessed for its ability to scavenge free radicals in four in vitro test models. Its ability to inhibit the activity of H+/K+-ATPase enzyme was also evaluated. Molecular docking was carried out on phytoconstituents, namely, vitexin, isovitexin, orientin, and isoorientin reported to be present in the whole plant extract. Results: Data obtained indicated that D. rotundifolia extract (DRE) exhibits strong antioxidant activity. DRE also showed inhibitory effects on H+/K+-ATPase enzyme activity. Docking studies affirmed the in vitro binding effect of the extract to H+/K+-ATPase. Conclusion: These findings suggest that the plant extract possess antioxidant and antipeptic ulcer activity.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed2001    
    Printed69    
    Emailed0    
    PDF Downloaded237    
    Comments [Add]    
    Cited by others 1    

Recommend this journal