Table of Contents    
RESEARCH ARTICLE
Year : 2015  |  Volume : 6  |  Issue : 1  |  Page : 163-168  

Association of atherosclerosis with dyslipidemia and co-morbid conditions: A descriptive study


1 Department of Medicine, GGS Medical College and Hospital, Faridkot, Punjab, India
2 Department of Radio Diagnosis, GGS Medical College and Hospital, Faridkot, Punjab, India
3 Department of Pharmacology, GGS Medical College and Hospital, Faridkot, Punjab, India
4 Department of Social and Preventive Medicine, GGS Medical College and Hospital, Faridkot, Punjab, India

Date of Web Publication14-Jan-2015

Correspondence Address:
Dr. Ravinder Garg
Department of Medicine, GGS Medical College and Hospital, Faridkot, Punjab
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0976-9668.149117

Rights and Permissions
   Abstract 

Background: Dyslipidemia (Dys), the commonest cause of cardiovascular diseases, leads to lipid deposits on the arterial wall, thereby aggravating the process of atherosclerosis. To assess the impact of Dys and other co-morbid conditions on atherosclerosis (i.e., increased intimo medial thickness (IMT) or plaques) in the common carotid arteries (CCAs) we studied the incidence and association of plaques and increased IMT in dyslipidemic patients from North Indian population (south-west of Punjab, India). Materials and Methods: A cross-sectional study consisting of 88 (male-39 and female-49) dyslipidemic patients (age group 30-80 years); was designed. Blood pressure, waist circumference (WC), hip circumference (HC) were measured and fasting lipid profile, renal function, and liver function tests were performed. B-mode sonography, for CCA, was performed to assess IMT. Results: Average value (mg/dl) of total cholesterol, triglyceride, low density lipoprotein and high density lipoprotein in males and females was 220.30 versus 231.93, 240.3 versus 242.14, 125.29 versus 133.62 and 44.33 versus 46.09 respectively (P > 0.05, all), while WC, HC, systolic blood pressure and diastolic blood pressure were 101.8 versus 96.53 cm, 98.23 versus 99.53 cm, 143.23 versus 138.98 mmHg and 91.53 versus 88.97 mmHg respectively. Increased IMT and atherosclerotic plaques were observed in 36.36% cases (n = 32, male - 14 and female - 18) and 29.54% cases (n = 26, male 14, female 12) respectively. Odd ratio (OR) for plaques was more for Dys with coronary artery disease (CAD; 11.43) and Dys with CAD (Dys-CAD) + hypertension (HT) (24) respectively vs isolated Dys. Conclusion: Dyslipidemia patients have higher waist and HCs than normal subjects. Incidence and OR of plaques is higher in Dys-CAD or Dys-CAD + HT when compared to isolated Dys or Dys with HT. Hence, treatment of dyslipidemic patients' needs to be intensified if more than one risk factor(s) is present simultaneously.

Keywords: Cardiovascular diseases, carotid intimo-medial thickness, common carotid arteries, co-morbid conditions, hip circumference, hypertension, risk factors, waist circumference


How to cite this article:
Garg R, Aggarwal S, Kumar R, Sharma G. Association of atherosclerosis with dyslipidemia and co-morbid conditions: A descriptive study. J Nat Sc Biol Med 2015;6:163-8

How to cite this URL:
Garg R, Aggarwal S, Kumar R, Sharma G. Association of atherosclerosis with dyslipidemia and co-morbid conditions: A descriptive study. J Nat Sc Biol Med [serial online] 2015 [cited 2020 Oct 22];6:163-8. Available from: http://www.jnsbm.org/text.asp?2015/6/1/163/149117


   Introduction Top


Cardiovascular disorders (CVD) are major noncommunicable diseases worldwide and atherosclerosis is the major risk factor for morbidity and mortality associated with CVD. [1],[2],[3] Dyslipidemia (Dys), cigarette smoking, hypertension (HT), diabetes, obesity and sedentary lifestyle are the modifiable risk factors for CVDs that promote atherosclerosis. [1],[2],[3],[4] Of these, Dys stands out prominently as it enhances the deposition of the fats in the arteries, thereby narrowing the lumen, obstructing the blood supply, leading to thrombo-embolic episode(s) such as stroke, transient ischemic attack (TIA), ischemic heart disease, pulmonary embolism, etc. [1],[2],[3],[4],[5],[6] Patients with CVDs have silent atherosclerotic plaque(s) or increased intimo-medial thickness (IMT) prior to the incidence of thrombo-embolic events. [1],[7] Due to genetic or environmental variations, Indians have different kinds of risk factors, [6],[8] with only a few studies been reported. Hence, this study was designed involving North Indian subjects from the Malwa-region of Punjab (near Indo-Pak Border of Punjab), wherein socioeconomic background and living standards are considerably different from western nations. The study was designed to explore the risk factors and their association with atherosclerosis (increased IMT or plaques), in patients with Dys. Other co-morbid conditions were also studied, and patients were categorized according to risk factors/co-morbid conditions for efficient clinical management. We aimed to see the incidence of plaques and increased IMT in dyslipidemic patients and association of these atherosclerotic changes with Dys and co-morbid diseases in North Indian population from the Malwa-region of Punjab.


   Materials and Methods Top


A cross-sectional study with participants recruited from Medicine Department (outpatients and inpatients) of Guru Gobind Singh Hospital attached to Guru Gobind Singh Medical College, Faridkot was designed. Subjects in the age group of 30-80 years, having Dys with or without HT or coronary artery disease (CAD), were enrolled during a period of 3 months. A patient was diagnosed dyslipidemic according to NCEP-ATP III criteria as below: [6] Total cholesterol (TC) >240 mg/dl or low density lipoprotein-cholesterol (LDL-C) >160 mg/dl or high density lipoproteins cholesterol (HDL-C) <40 mg/dl (male) or <50 mg/dl (female) or triglycerides (TGs) >200 mg/dl.

Nursing mothers or pregnant subjects, or subjects having hepatitis or cirrhosis of the liver, bronchial asthma, HIV, tuberculosis, chronic inflammatory disease, thyroid disorders, diabetes mellitus, renal failure or on corticosteroids, oral contraceptive pills, hormonal replacement therapy, alcoholism, tobacco chewing or smoking were excluded from the study. A total of 88 out of 100 individuals with Dys underwent physical examination and ultrasonography of the carotid arteries. We excluded 12 individuals who didn't fit the inclusion criteria. All individuals underwent a detailed medical history by a standardized questionnaire to obtain information about current and past medication(s) use, presence of CVDs (HT, CAD, stroke, TIA, etc.) and habits of smoking and alcohol. Patients underwent general physical examination including measurement of waist circumference (WC), Hip circumference (HC) and systemic blood pressure of both arms (systolic and diastolic, taken by a sphygmomanometer). Under aseptic conditions venous blood was collected after overnight fasting for lipid profile, serum glucose, blood urea, serum creatinine and other tests [Figure 1]. All the B-mode ultrasonographic measurements of common carotid arteries (CCAs) and internal carotid arteries were performed by a single blinded expert reader. The Institutional Ethical Committee approved the protocol, and all the subjects gave written informed consent before participating in the study.
Figure 1: Study design

Click here to view


Carotid duplex sonographic of common carotid arteries

Carotid ultrasonography followed by a duplex color Doppler examination was performed on patients in the supine position with Philips Envisor unit with a linear probe of 7.5-12 MHz. Both left and right CCA were analyzed in longitudinal and transverse planes. IMT was measured at mid-CCA, about 10 mm proximal to the carotid bulb, between the leading edge of first echogenic line (lumen intima interface) and second echogenic line (upper layer of adventitia), in the far and near walls of the artery [Figure 2]. [9],[10] The mean value of three readings in the mid-segment of CCA was recorded as IMT. A plaque is defined as a focal structure that encroaches into the arterial lumen of at least 0.5 mm or 50% of the surrounding IMT value or demonstrates a thickness >15 mm as measured from the media-adventitia interface to the intima lumen interface [Figure 3], and an IMT of <8 mm as normal while >8 mm considered to be increased IMT [Figure 4]. [9]
Figure 2: Carotid sonography showing normal intimo-medial thickness

Click here to view
Figure 3: Carotid sonography showing plaque at near wall at bifurcation

Click here to view
Figure 4: Carotid sonography showing increased intimo-medial thickness

Click here to view


Statistical analysis

Data are represented as mean and standard deviation for continuous variable and frequency and column percentages for categorical variables. Participant's demography was stratified by gender and Student's t-test applied. Data were categorized into four groups according to risk factors/co-morbid conditions. The Chi-square test and Fischer exact test for categorical data were used to evaluate the association between variables. P < 0.05 was considered to be significant.


   Results Top


The average age of the study population was 55.14 ± 9.25 years (male-55.89 ± 10.92 and female-54.55 ± 7.74). Detailed clinical characteristics of the patients are listed in [Table 1]. As per the observations the patients were categorized into four groups that are, patients with isolated Dys, Dys with HT (Dys-HT), Dys with CAD (Dys-CAD), and Dys-HT and CAD (Dys-HT-CAD) [Table 2]. [Table 2] shows mean and standard deviation of age, TC, TGs, LDL, VLDL, HDL, WC and HC, systolic blood pressure (SBP) and diastolic blood pressure (DBP), number of plaques and IMT in these categories. Average value (mg/dl) of TC, TG, LDL and HDL in males and females was 220.30 versus 231.93, 240.3 versus 242.14, 125.29 versus 133.62 and 44.33 versus 46.09 respectively (P > 0.05, all), whereas WC, HC, SBP and DBP were 101.8 versus 96.53 cm, 98.23 versus 99.53 cm, 143.23 versus 138.98 mmHg and 91.53 versus 88.97 mmHg respectively [Table 1]. Increased IMT was seen in 36.36% cases (n = 32, male-14 and female-18) while plaques in 29.54% cases (n = 26, male-14, female-12) [Table 1]. Odds ratio (OR) for plaques was 24.00 (P < 0.01) for Dys-HT-CAD patients when compared to isolated dyslipidemic patients. Incidence of plaque and OR was significantly higher in the Dys-HT-CAD category when compared to Dys, Dys-HT and Dys-CAD categories [Table 3]. Plaques in CCA were observed in 5.9% subjects with Dys, 25% with Dys-HT, 41.7% with Dys-CAD, and 60% with Dys-HT-CAD [Table 4] indicating that CCA plaques are higher in dyslipidemic subjects having multiple co-morbid conditions vis-a-vis Dys alone or Dys with a single co-morbid disease.
Table 1: Baseline characteristics of the population

Click here to view
Table 2: Categorization of the participants according to risk factors/co-morbid conditions

Click here to view
Table 3: Risk of plaque formation among subgroups of dyslipidemia

Click here to view
Table 4: Different co-morbid conditions and plaques in common carotid arteries

Click here to view



   Discussion Top


All the patients in our study group had higher average WC compared to normal values (male-90 cm and female-80 cm, respectively). [11] Average age of male and female subjects in our study group was higher [8] and lower [12] compared with previous reports. However, our data is consistent with the previous report suggesting a higher incidence of Dys and other risk factors at an early age of their life as compared to the Western population. Genetic factors or lifestyle may be major factors responsible for such early Dys events which are a cause for concern. Further the average age of patients in isolated Dys (50.06 ± 8.89 years) was less as compared to Dys-HT (55.6 ± 9.08 years), Dys-CAD (57.64 ± 8.23 years) and Dys-HT-CAD (58.73 ± 10 years) respectively, indicating an early role of Dys in progression of cardiovascular pathology. The increased blood pressure observed in our study group is consistent with previous reports. [13} Both SBP and DBP was higher in patients with Dys-HT (147.3 ± 21.95 and 94 ± 13.3) and Dys-HT-CAD (144.4 ± 15.06 and 93.6 ± 6.74) as compared to Dys (131.9 ± 17.21 and 83.1 ± 8.73), Dys-CAD (123.8 ± 17.67 and 80.9 ± 13.8) respectively. Thus, further supporting the primary role of Dys in development of cardiovascular hemodynamics. The plasma levels of TC and LDL was less, while TGs was higher in our study group, which is consistent with the previous report [13} and suggests that our study group is more prone to hypertriglyceridaemia than hypercholesterolemia in contrast to the Western population.

The mean value of carotid IMT in our study group was higher as compared to previous reports from Korea, [14] Japan, [14] and Mexican American population. [15] However, the IMT was lower compared to previous reports from the western population. [13],[16] Plaques in CCA were observed in 29.54% cases in the present study and were both lower [13] and higher [16] than previous reports from the western population. TC, LDL, WC and HC were highest in Dys-HT-CAD patients as compared to other categories while TGs and VLDL-C levels were higher in Dys patients as compared to Dys-CAD (242 ± 62.24), Dys-HT (235.5 ± 74.81) and Dys-HT-CAD (224.5 ± 51.7) respectively. Dys-HT-CAD patients had a higher percentage of plaques, WC and HC as compared to Dys-CAD, Dys-HT and isolated Dys respectively. OR and incidence of plaques were significantly higher in the Dys-CAD-HT and Dys-CAD categories as compared to Dys and Dys-HT categories, respectively. Our observations suggest that plaques have a strong association with co-morbid conditions viz. Dys-HT-CAD and this association becomes progressive when more than one co-morbid condition exists simultaneously in the dyslipidemic patient. ACC/AHA Cardiovascular Risk Assessment Guidelines (2013) [5] recommend hs-C-reactive protein, coronary artery calcium score, microalbuminuria, etc. as better tools for risk assessment of CVDs, but in developing countries like India because of the cost and limited availability of these investigations, ultrasonographic assessment of IMT and plaques in the arteries is a cost effective and easily available modality for risk assessment of dyslipidemic patients. Limitations of the study: As the study design was cross-sectional, and hospital based, performed in a relatively small sample size of patients, the results need to be confirmed in a large, population based study. Nevertheless dyslipidemic patients should be explored for other CVD risk factors such as HT, obesity, increased IMT and plaques in CCA with noninvasive B-mode Ultrasonography for early detection of progressive cardiovascular pathology, which can help initiate early therapy to prevent development of serious CVDs complications and improve therapeutic outcome.

In conclusion, dyslipidemic patients have higher waist and HCs. Incidence and OR of plaques is higher in patients who have Dys-CAD or. Dys-HT-CAD as compared to those having isolated Dys or Dys-HT. Hence, dyslipidemic patients should be treated accounting for the existence of simultaneous risk factor(s).

 
   References Top

1.
Libby P. The pathogenesis of atherosclerosis. In: Kasper DL, Braunwald E, Fauci AS, Hauser SL, Longo DL, Jameson JL, editors. Harrison′s Principles of Internal Medicine. 16 th ed. New York: McGraw-Hill; 2005. p. 1425-30.  Back to cited text no. 1
    
2.
Schoen FJ. Blood Vessels. Robbins Pathologic Basis of Disease. 5 th ed. Philadelphia: W.B Saunders; 1994. p. 467-516.  Back to cited text no. 2
    
3.
WHO. Non-communicable diseases. Cardiovascular Diseases (CVDs). Available from: http://www.who.int/mediacentre/factsheets/fs317/en/#. [Last accessed on 2014 May 10, at 9:40 pm].  Back to cited text no. 3
    
4.
Talbert RL. Dyslipidemia. In: Diprio JT, Talbert RL, Yee GC, Matzke GR, Wells BG, Posey LM, editor. Pharmacotherapy - A Pathophysiological Approach. 8 th ed. New York: McGraw-Hill; 2008. p. 365-88.  Back to cited text no. 4
    
5.
Goff DC, Lloyd-Jones DM, Bennett G, Coady S, D′Agostino RB, Gibbons R, et al. 2013 ACC/AHA Guideline on the Assessment of Cardiovascular Risk: A report of the American College of Cardiology/American Heart Association Task Force on Practice Guidelines. JACC 2014;63:2935-59. Available from: http://www.content.onlinejacc.org/article.aspx?articleid=1879711. [Last accessed on 2014 Aug 29, at 10:00 am].  Back to cited text no. 5
    
6.
National Cholesterol Education Program (NCEP). Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panal III). Third Report of The National Cholesterol Education Program (NCEP) Expert Panel on detection, Evaluation and Treatment of High Blood Cholesterol in adults (Adult Treatment Panel III) final report. Circulation 2002;106:3143-421.  Back to cited text no. 6
    
7.
Hurst RT, Ng DW, Kendall C, Khandheria B. Clinical use of carotid intima-media thickness: Review of the literature. J Am Soc Echocardiogr 2007;20:907-14.  Back to cited text no. 7
    
8.
Bhopal R, Unwin N, White M, Yallop J, Walker L, Alberti KG, et al. Heterogeneity of coronary heart disease risk factors in Indian, Pakistani, Bangladeshi, and European origin populations: Cross sectional study. BMJ 1999;319:215-20.  Back to cited text no. 8
    
9.
Touboul PJ, Hennerici MG, Meairs S, Adams H, Amarenco P, Desvarieux M, et al. Mannheim intima-media thickness consensus. Cerebrovasc Dis 2004;18:346-9.  Back to cited text no. 9
    
10.
Lim TK, Lim E, Dwivedi G, Kooner J, Senior R. Normal value of carotid intima-media thickness - a surrogate marker of atherosclerosis: Quantitative assessment by B-mode carotid ultrasound. J Am Soc Echocardiogr 2008;21:112-6.  Back to cited text no. 10
    
11.
India Reworks Obesity Guidelines, BMI lowered. Available from: http://www.igovernment.in/igov/news/26259/india-reworks-obesity-guidelines-bmi-lowered. [Last accessed on 2014 Aug 30, at 7:55 am].  Back to cited text no. 11
    
12.
The Atherosclerosis Risk in Communities (ARIC) Study: Design and objectives. The ARIC investigators. Am J Epidemiol 1989;129:687-702.  Back to cited text no. 12
    
13.
Herder M, Arntzen KA, Johnsen SH, Mathiesen EB. The metabolic syndrome and progression of carotid atherosclerosis over 13 years. The Tromsø study. Cardiovasc Diabetol 2012;11:77.  Back to cited text no. 13
    
14.
Choo J, Ueshima H, Jang Y, Sutton-Tyrrell K, El-Saed A, Kadowaki T, et al. Difference in carotid intima-media thickness between Korean and Japanese men. Ann Epidemiol 2008;18:310-5.  Back to cited text no. 14
    
15.
Laing ST, Smulevitz B, Vatcheva KP, Rentfro AR, McPherson DD, Fisher-Hoch SP, et al. High prevalence of subclinical atherosclerosis by carotid ultrasound among Mexican Americans: Discordance with 10-year risk assessment using the framingham risk score. Echocardiography 2012;29:1224-32.  Back to cited text no. 15
    
16.
Bots ML, Hoes AW, Koudstaal PJ, Hofman A, Grobbee DE. Common carotid intima-media thickness and risk of stroke and myocardial infarction: The Rotterdam Study. Circulation 1997;96:1432-7.  Back to cited text no. 16
    


    Figures

  [Figure 1], [Figure 2], [Figure 3], [Figure 4]
 
 
    Tables

  [Table 1], [Table 2], [Table 3], [Table 4]


This article has been cited by
1 Serum vitamin E as a significant prognostic factor in patients with dyslipidemia disorders
Maral Barzegar-Amini,Hamideh Ghazizadeh,Seyed Mohammad reza Seyedi,Hamid Reza Sadeghnia,Akram Mohammadi,Mahdi Hassanzade-Daloee,Elham Barati,Sara kharazmi-Khorassani,jasmin kharazmi-Khorassani,Maryam Mohammadi-Bajgiran,Shima Tavallaie,Gordon A. Ferns,Mohsen Mouhebati,Mahmoud Ebrahimi,Maryam Tayefi,Majid Ghayour-Mobarhan
Diabetes & Metabolic Syndrome: Clinical Research & Reviews. 2019; 13(1): 666
[Pubmed] | [DOI]
2 Dyslipidemia Associated with Hypertension Increases the Risks for Coronary Heart Disease: A Case-Control Study in Harapan Kita Hospital, National Cardiovascular Center, Jakarta
Rea Ariyanti,Besral Besral
Journal of Lipids. 2019; 2019: 1
[Pubmed] | [DOI]
3 Lanosteryl triterpenes from Protorhus longifolia as a cardioprotective agent: a mini review
Nonhlakanipho F. Sangweni,Phiwayinkosi V. Dludla,Rebamang A. Mosa,Abidemi P. Kappo,Andy Opoku,Christo J. F. Muller,Rabia Johnson
Heart Failure Reviews. 2018;
[Pubmed] | [DOI]
4 Lipid lowering in dialysis patients with cardiovascular disease who are awaiting kidney transplantation
Fahad Aziz,Neetika Garg,Sandesh Parajuli,Arjang Djamali,James H. Stein,Didier Mandelbrot
Clinical Transplantation. 2018; : e13452
[Pubmed] | [DOI]
5 High fat diet modifies the association of lipoprotein lipase gene polymorphism with high density lipoprotein cholesterol in an Asian Indian population
K. A. Ayyappa,I. Shatwan,D. Bodhini,L. R. Bramwell,K. Ramya,V. Sudha,R. M. Anjana,J. A. Lovegrove,V. Mohan,V. Radha,K. S. Vimaleswaran
Nutrition & Metabolism. 2017; 14(1)
[Pubmed] | [DOI]



 

Top
  
 
  Search
 
    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Access Statistics
    Email Alert *
    Add to My List *
* Registration required (free)  

 
  In this article
    Abstract
   Introduction
    Materials and Me...
   Results
   Discussion
    References
    Article Figures
    Article Tables

 Article Access Statistics
    Viewed1933    
    Printed48    
    Emailed0    
    PDF Downloaded228    
    Comments [Add]    
    Cited by others 5    

Recommend this journal