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  Indian J Med Microbiol
 

Figure 6: The protective effects of ANTs on the H2O2-induced expression of the autophagy-related LC3-II gene. (a) reverse transcription-polymerase chain reaction of LC3-II in H2O2-stimulated fibroblasts without or with ANT pretreatment. Scale bar: 50 μm (lower magnification images) and 25 μm (higher magnification images). (b) Fluorescence microscopy of LC3-II protein in H2O2-stimulated fibroblasts with or without anthocyanins. (c) The percentages of puncta-positive cells (>10 puncta/cell) out of the total number of fluorescently stained cells. The data are from four independent experiments. *P < 0.05, and **P < 0.01

Figure 6: The protective effects of ANTs on the H<sub>2</sub>O<sub>2</sub>-induced expression of the autophagy-related LC3-II gene. (a) reverse transcription-polymerase chain reaction of LC3-II in H<sub>2</sub>O<sub>2</sub>-stimulated fibroblasts without or with ANT pretreatment. Scale bar: 50 μm (lower magnification images) and 25 μm (higher magnification images). (b) Fluorescence microscopy of LC3-II protein in H<sub>2</sub>O<sub>2</sub>-stimulated fibroblasts with or without anthocyanins. (c) The percentages of puncta-positive cells (>10 puncta/cell) out of the total number of fluorescently stained cells. The data are from four independent experiments. *<i>P</i> < 0.05, and **<i>P</i> < 0.01