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  Indian J Med Microbiol
 

Figure 4: Protective effects of ANTs on H2O2-induced apoptosis. rat dermal fibroblasts were treated with 10 or 25 μg/mL crude ANT and then exposed to H2O2. (a) DNA condensation was detected by 4′,6-diamidino-2-phenylindole staining. Regions of condensed chromatin appear as dense blue areas (white arrows). Scale bar: 100 μm (low magnification images) and 50 μm (high magnification images). (b) The graph presents the percentage of apoptotic cells out of the total cells. The results are expressed as the mean ± standard deviation of three independent experiments. * P < 0.05, ** P < 0.01 and ***P < 0.001

Figure 4: Protective effects of ANTs on H<sub>2</sub>O<sub>2</sub>-induced apoptosis. rat dermal fibroblasts were treated with 10 or 25 μg/mL crude ANT and then exposed to H<sub>2</sub>O<sub>2</sub>. (a) DNA condensation was detected by 4′,6-diamidino-2-phenylindole staining. Regions of condensed chromatin appear as dense blue areas (white arrows). Scale bar: 100 μm (low magnification images) and 50 μm (high magnification images). (b) The graph presents the percentage of apoptotic cells out of the total cells. The results are expressed as the mean ± standard deviation of three independent experiments. * <i>P</i> < 0.05, ** <i>P</i> < 0.01 and ***<i>P</i> < 0.001