Figure 4: (a) Multiple sequence alignment of EoKAS-II and EgTKAS-II amino acid sequences with their selected counterparts from other organism. Protein sequences were aligned using the CLUSTALW alignment algorithm. The three active sites (residues) of EoKAS-II and EgTKAS-II proteins corresponding to E. coli KAS, Cys163, His298, and His333 are highlighted in pink color; the numbers at top indicate the residue ruler for whole alignment. GenBank accession numbers are as follows: Eo, FJ940767; EgT, AF220453; Jc, DQ987700; Gh, HM236494; Rc, EQ973802; Ah, EU823327; Cw, U67317; Pf, AF026149; Gm, AY907523; Ha, DQ835562; Bn, AF244520; Cr, DS496157; Ec, 1FJ8_A. Abbreviations: Eo, Elaeis oleifera; EgT, Elaeis guineensis (Tenera); Jc, Jatropha curcas; Gh, Gossypium hirsutum; Rc, Ricinus communis; Ah, Arachis hypogaea; Cw, Cuphea wrightii; Pf, Perilla frutescens; Gm, Glycine max; Ha, Helianthus annuus; Bn, Brassica napus; Cr, Chlamydomonas reinhardtii; Ec, Escherichia coli. This alignment is produced by CLUSTAL W multiple sequence alignment program. (b) Radial phylogenetic tree of amino acid sequence relationship among the proteins aligned in (a). The phylogenetic distance scale at bottom is proportional to the divergence between the sequences.