Close
  Indian J Med Microbiol
 

Figure 2: Nucleotide and deduced amino acid sequences of E. guineensis (Tenera) ƒÒ-ketoacyl-[ACP] synthase II (EgTKAS-II) cDNA clone. An open reading frame and noncoding regions are shown in capital and small letters, respectively. Nucleotides shown in bold brown color represent the location of the forward primer (3'-RACE FP) used in 3'-RACE and reverse primer (5'-RACE RP) used in 5'-RACE. Arrows indicate the 5'to 3' direction of the primer. Nucleotides shown in brown color represent the overlapping regions with cDNA fragments isolated using 5' and 3'-RACE. The deduced amino acid sequence is given below the nucleotide sequence, which is numbered at the both ends of each sequence line. The open reading frame encodes for a protein of 573 amino acid residues. Amino acid residues are numbered beginning with the initial Methionine (m) till last Cysteine (c) residue. Initiation and termination codons are shown in green and red color, respectively. The 'ƒÒ-ketoacyl-[ACP] synthase (KAS) (type I and II)', 'Elongating' condensing enzymes and 'Condensing enzymes super-family conserved domain (multi-domain) residues (174 to 546) are highlighted in green color. Residues (1 to 563) underlined with pink color line are part of the '3-oxoacyl-[ACP] synthase II' domain (region name="PLN02787"). *represent the termination codon. This cDNA was isolated from E. guineensis 17-week-old fruit-mesocarp tissue by using RT-PCR, and 5' and 3' RACE techniques.

Figure 2: Nucleotide and deduced amino acid sequences of E. guineensis (Tenera) ƒÒ-ketoacyl-[ACP] synthase II (EgTKAS-II) cDNA clone. An open reading frame and noncoding regions are shown in capital and small letters, respectively. Nucleotides shown in bold brown color represent the location of the forward primer (3'-RACE FP) used in 3'-RACE and reverse primer (5'-RACE RP) used in 5'-RACE. Arrows indicate the 5'to 3' direction of the primer. Nucleotides shown in brown color represent the overlapping regions with cDNA fragments isolated using 5' and 3'-RACE. The deduced amino acid sequence is given below the nucleotide sequence, which is numbered at the both ends of each sequence line. The open reading frame encodes for a protein of 573 amino acid residues. Amino acid residues are numbered beginning with the initial Methionine (m) till last Cysteine (c) residue. Initiation and termination codons are shown in green and red color, respectively. The 'ƒÒ-ketoacyl-[ACP] synthase (KAS) (type I and II)', 'Elongating' condensing enzymes and 'Condensing enzymes super-family conserved domain (multi-domain) residues (174 to 546)
are highlighted in green color. Residues (1 to 563) underlined with pink color line are part of the '3-oxoacyl-[ACP] synthase II' domain (region name=