Journal of Natural Science, Biology and Medicine

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 10  |  Issue : 2  |  Page : 157--166

Antioxidant and cytotoxic potential of leaf extracts of Costus igneus


Waseem Iqbal Khanday, Nazir Ahmad Wani, Balaji Paulraj 
 PG and Research Centre in Biotechnology, MGR College, Hosur, Tamil Nadu, India

Correspondence Address:
Balaji Paulraj
PG and Research Centre in Biotechnology, MGR College, Hosur - 635 130, Tamil Nadu
India

Background: Costus igneus leaves are traditionally used to treat diabetes and this plant is commonly known as insulin plant. Hypothesis: The purpose of this study was to evaluate the antioxidant and cytotoxic potential of different leaf extracts of C. igneus. Materials and Methods: The antioxidant potential of various leaf extracts of C. igneus was established by ferric reducing antioxidant power (FRAP) assay, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay, metal-chelating activity, phosphomolybdenum assay, superoxide radical scavenging activity, 2,2-diphenylpicrylhydrazyl (DPPH) assay, reducing power assay, and cytotoxic activity by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on breast cancer cell line Michigan Cancer Foundation (MCF-7). Results: In FRAP assay, acetone extract showed higher activity of 276.31 mmol Fe (II) E/mg extract. In ABTS assay, hexane extract showed the highest scavenging activity of 12878.893 μM Trolox equivalent antioxidant capacity (TEAC)/g extract. Hot water extract registered higher metal ion-chelating ability with 2.94 mg ethylenediaminetetraacetic acid equivalent/g extract. Hexane extract has recorded the highest total antioxidant capacity of 65.00 mg AAE/mg in phosphomolybdenum assay. Superoxide radical scavenging activity was found maximum in acetone extract with 41.11%. In DPPH assay, acetone extract recorded total free radical scavenging capacity with IC50 value of 40.70 μg/ml. Hexane extract has recorded significant highest reducing power at concentration 100 μg/μl with maximum optical density of 0.177. Acetone extract of C. igneus leaf extract at concentration of 150 μg/ml showed the highest cytotoxic activity on cancer cell line with cell viability of 65.51%. Conclusion: The present study explored that C. igneus shows efficient antioxidant activity as well as cytotoxic activity and could act as safe and cost-effective with potential biological applications.


How to cite this article:
Khanday WI, Wani NA, Paulraj B. Antioxidant and cytotoxic potential of leaf extracts of Costus igneus.J Nat Sc Biol Med 2019;10:157-166


How to cite this URL:
Khanday WI, Wani NA, Paulraj B. Antioxidant and cytotoxic potential of leaf extracts of Costus igneus. J Nat Sc Biol Med [serial online] 2019 [cited 2020 Feb 21 ];10:157-166
Available from: http://www.jnsbm.org/article.asp?issn=0976-9668;year=2019;volume=10;issue=2;spage=157;epage=166;aulast=Khanday;type=0