BIOLOGY
Year : 2010  |  Volume : 1  |  Issue : 1  |  Page : 16-21

Intracellular scavenging activity of Trolox (6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid) in the fission yeast, Schizosaccharomyces pombe


1 Department of Biochemistry, Upper Nile University, P.O. Box 1660 Khartoum, Sudan
2 Department of Molecular Biology and Genetics, Istanbul University, Vezneciler 34118, Istanbul, Turkey

Correspondence Address:
Ismail Hamad
Department of Biochemistry, Upper Nile University, Faculty of Medicine and Health Sciences, P.O. Box 1660 Khartoum
Sudan
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0976-9668.71667

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The ability of Trolox (6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid), a water-soluble vitamin E analogue, to prevent oxidative damages is well characterized, but the mechanisms underlying it remain unclear. The protective effect of Trolox pre-treatment on H 2 O 2 -induced toxicity might be attributed to the decreased cellular permeability to H 2 O 2 or in vitro scavenging activity of Trolox, induction of antioxidant enzymes or the direct scavenging activity of Trolox. The results obtained rule out the first and second possibilities and intracellular scavenging activity was found to be the mechanism whereby Trolox confers protection. This was confirmed by measuring protein oxidation (levels), and the observed decrease in proteasomal activity indicated that the decrease in protein carbonyls was due to Trolox scavenging activity rather than proteasome activation. In conclusion, the intracellular scavenging activity of Trolox is a key protective mechanism against H 2 O 2 . These findings obtained in Schizosaccharomyces pombe, a good model organism for eukaryotic cells, can be used as standard protocols for investigating the antioxidant activity of pure or complex potential antioxidants.


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